The particular time of day doesn't matter -- whenever works best for you to incorporate it into a daily routine. Consistency is most important. That said, as a whole food, ARMRA Colostrum is typically well-tolerated. You can safely increase to multiple servings either all at once or spaced throughout the day for amplified effect, as the benefits increase as more servings are taken. You can see how you feel and what works for you. Most users enjoy 2-4 servings a day.